Evidence that more than 90% of beta-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus

Yamashita, T.; Iida, A.; Morikawa, H.

Evidence that more than 90% of beta-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus

1991

Yamashita, T. | Iida, A. | Morikawa, H.

Plasmid DNA harboring the beta-glucuronidase (GUS) gene, coated on gold particles, was delivered into cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells using a pneumatic particle gun. Cytological analyses of intracellular location of the introduced gold particles before and after GUS-expression assay indicated that more than 90% of GUS-expressing cells after bombardment received a DNA-coated particle in their nucleus.

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AGROVOC关键词

enzyme activity gene expression gene transfer nicotiana tabacum spatial distribution

书目信息

发表于

Plant physiology

卷 97 期 2 页码 829 - 831 ISSN 0032-0889

其它主题

Beta-glucuronidase; Cell suspension culture

语言

英语

类型

Journal Article; Text

自何时收录于AGRIS: 2024-02-28

修改日期: 2026-02-03

格式: MODS

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Evidence that more than 90% of beta-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus

1991

AGROVOC关键词

书目信息