Dimethyl sulphoxide (DMSO) and dimethyl sulphide (DMS) as inhibitors of methane oxidation in forest soil
2003
Saari, A. | Martikainen, P.J.
Dimethyl sulphoxide (DMSO) at 14 mM inhibits CH4 oxidation in forest soil, but the inhibition mechanism is unknown. When soil slurries are incubated in gas tight flasks, there is a lag of about 45 h before DMSO inhibits CH4 oxidation. We tried to determine if some metabolic compound derived from DMSO, as a result of microbial activity, is responsible for the inhibition. Dimethyl sulphide (DMS) accumulated in the sealed flasks up to 5-83 microliter l-1 in the headspace during a 2-week period. DMS at 1 microliter l-1 in the headspace (0.64 micromolar in soil-water slurry) had a negligible effect on CH4 oxidation but 50 microliter l-1 DMS (32 micromolar) inhibited CH4 oxidation completely. However, the inhibition by DMSO was already evident after 45 h, when DMS concentrations were generally non-inhibiting (0.1-0.7 microliter l-1). DMSO was also shown to inhibit CH4 oxidation when the DMS produced was continuously removed. Results suggest that the production of DMS from DMSO makes a minor contribution to the inhibition of CH4 oxidation by DMSO with incubation times relevant in CH4 oxidation studies.
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