The formation of Cd-phytochelatin complexes in plant cell cultures
1997
Kneer, R. | Zenk, M.H.
Three distinct Cd-phytochelatin complexes were purified to apparent homogeneity by gel filtration on Sephadex G50 of crude Cd-phytochelatin preparations isolated from Cd-treated suspension cultures of Rauvolfia serpentina. These purified complexes were analysed and found to be significantly different in PC chainlength, S:Cd ratio, and sulphide content. Kinetic measurements of PC(n) and sulphide content in Cd-treated suspension cultures of R. serpentina and Silene cucubalus showed that there are two possible ways of decreasing the amount of sulphur (and nitrogen) needed for Cd2+ sequestration: either by increasing the PC chainlength or by incorporating sulphide into the complexes. The latter process appears to be the more effective.
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