Molecular characterization of canine kobuvirus in wild carnivores and the domestic dog in Africa
2015
Olarte-Castillo, Ximena A | Heeger, Felix | Mazzoni, Camila J | Greenwood, Alex D | Fyumagwa, Robert | Moehlman, Patricia D | Hofer, Heribert | East, Marion L
Knowledge of Kobuvirus (Family Picornaviridae) infection in carnivores is limited and has not been described in domestic or wild carnivores in Africa. To fill this gap in knowledge we used RT-PCR to screen fresh feces from several African carnivores. We detected kobuvirus RNA in samples from domestic dog, golden jackal, side-striped jackal and spotted hyena. Using next generation sequencing we obtained one complete Kobuvirus genome sequence from each of these species. Our phylogenetic analyses revealed canine kobuvirus (CaKV) infection in all four species and placed CaKVs from Africa together and separately from CaKVs from elsewhere. Wild carnivore strains were more closely related to each other than to those from domestic dogs. We found that the secondary structure model of the IRES was similar to the Aichivirus-like IRES subclass and was conserved among African strains. We describe the first CaKVs from Africa and extend the known host range of CaKV.
显示更多 [+] 显示较少 [-]