Enzymes produced by soil fungi followed microaerobic growth on lignocellulosic materials
1999
Pavarina, E.C. | Sette, L.D. | Anazawa, T.A. | Durrant, L.R.
Four fungal strains able to grow under low oxygenation conditions were selected and used in studies to determine the production of enzymes (endoglucanases, exoglucases, beta-glucosidase, and peroxidases) that promote the degradation of lignocellulosic materials. The capacity of the fungi to ferment lignocellulosic materials was also investigated. Avicel, xylan, Whatman no. 1 filter paper, or agroindustrial residues were used as carbon sources in a medium containing mineral salts, vitamins, and cysteine as a reducing agent, under either microaerophilic or combined conditions (aerobic followed by microaerophilic conditions). The results obtained with strains Q10, H2, and LH5 suggest that they prefer a low oxygen concentration for growth and enzyme production. However, strain F20 seems to need higher levels of oxygenation. Lignocellulolytic activities were detected in all strains but varied with the carbon source used for growth. In general, the highest levels of these activities were produced by strain H2 under microaerophilic conditions. Ethanol and other nongaseous fermentation products were detected following high-performance liquid chromatography analysis using a Supelcogel C-610H column, demonstrating the fermentative capability of these strains.
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