Bringing up small oocytes to eggs in pigs and cows
2003
Miyano, T.
It has been known that the mammalian ovary contains a huge number of non-growing small oocytes, of which only a small number grow to their final size, mature, and are ovulated. Artificial maturation of small oocytes could provide a new source of mature eggs for livestock production and assisted reproduction in humans and in endangered species. Two methods have been used for oocyte growth, in vitro growth (IVG) culture and xenotransplantation. By these methods, oocytes in some species grow up to their final size and acquire developmental competence, although the methods are still at the experimental stage. The experiments remind us of many basic questions in mammalian oogenesis: Does the oocyte require certain stimuli to initiate growth? How are the few oocytes selected to grow to final size? How do they grow up in follicular units? How do they acquire meiotic competence during the growth phase? This paper will give some clues to answer these questions by presenting our recent data from IVG and xenotransplantation experiments, and by illustrating differences between the oocytes of mice and larger animals.
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