Effects of bisphenol A on antioxidant system in soybean seedling roots
2015
Wang, Qingqing | Wang, Lihong | Han, Ruobing | Yang, Linying | Zhou, Qing | Huang, Xiaohua
Bisphenol A (BPA), an emerging pollutant in the environment, has potential toxic effects on plants. The toxicity mechanism, however, remains largely unknown. The antioxidant system plays an important role in protecting plants against the damage of stress. The present study investigated the effects of BPA on the antioxidant system (superoxide dismutase [SOD], peroxidase [POD], catalase [CAT], ascorbic acid [AsA], proline, reduced glutathione [GSH]), reactive oxygen species (ROS; hydrogen peroxide [H₂O₂], superoxide anion [O₂–]) accumulation, and membrane lipid peroxidation (malondialdehyde [MDA], cell membrane permeability) in soybean seedling roots. The 1.5 mg L⁻¹BPA exposure did not affect test indices in the roots. Exposure to 3.0 mg L⁻¹, 6.0 mg L⁻¹, 12.0 mg L⁻¹, or 24.0 mg L⁻¹BPA caused increases in SOD (except for 3.0 mg L⁻¹BPA) and CAT activities, as well as in AsA, proline, and GSH (except for 3.0 mg L⁻¹BPA) content, leading to increases in the H₂O₂and O₂–content and to membrane lipid peroxidation. Exposure to 48.0 mg L⁻¹or 96.0 mg L⁻¹BPA caused decreases in the CAT activity and AsA/GSH content, as well as increases in the SOD and POD activities and the proline content, leading to excess ROS accumulation (i.e., H₂O₂and O₂–) and cell membrane damage. After withdrawal of BPA exposure, ROS accumulation and membrane lipid peroxidation were alleviated by regulating a special antioxidant enzyme or substance. Environ Toxicol Chem 2015;34:1127–1133. © 2015 SETAC
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