The relation between in vivo ethylene production and oxygen partial pressure
2003
Sanders, Mark G. | Wild, Hans P.J de
Modelling in vivo ethylene production rate in relation to O2 partial pressure was used to improve understanding of enzyme kinetics of 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase). Tomato fruit were stored in an extensive range of O2 partial pressures at 8, 13 and 18 °C. Ethylene production was related to O2 partial pressure using a model based on Michaelis–Menten enzyme kinetics including a term resembling cooperativity. This cooperative model explains the measured data better than two models that were previously used: a logistic model and a model based on Michaelis–Menten kinetics. The model was also used for pear fruit, which had been stored for 0, 13 and 25 weeks. The high accuracy of the cooperative model indicates that ACC oxidase in vivo does not always obey simple Michaelis–Menten kinetics. The possibility of true cooperativity is discussed.
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