Genetic variation in raffinose family oligosaccharides and sucrose content in blackgram [Vigna mungo L. (Hepper)]
2014
Souframanien, J. | Roja, G. | Gopalakrishna, T.
Twenty four black gram genotypes were analysed for sucrose and RFOs content with an intention of identifying genotypes with low RFOs content. Analysis of raffinose family oligosaccharides revealed significant variation (P0.05) among the black gram genotypes. Of the 24 genotypes analysed, higher sucrose content was recorded in TU-72 (32.87 mg/g seed meal) followed by TU-26 (27.25 mg/g seed meal). Verbascose was the major RFO followed by stachyose among the black gram genotypes studied. Ajugose was a minor sugar followed by raffinose. The total RFOs concentration in black gram ranged from 26.64 to 61.57 mg/g with an average of 43.6 mg/g. The highest total RFOs content was observed in the genotype LBG-17 (61.57 mg/g). Low content of total RFOs was observed in TU43-1 (26.64mg/g). Wide variation was observed for individual RFOs such as raffinose, stachyose, verbascose, and ajugose, which ranged from 0.18 to 8.08mg/g, 8.90 to 37.27mg/g, 13.95 to 31.02 mg/g and 0.30 to 4.48 mg/g respectively. TU43-1 and LBG402 which recorded low content of total RFOs also showed lowest verbascose and stachyose content respectively. TU1-8201-5 recorded high verbascose (31.02 mg/g) with low stachyose (11.13 mg/g) and raffinose (0.18 mg/g) content. Significant correlation (P≤0.05) was detected among the four individual oligosaccharides with the total RFOs (r = 0.53, r = 0.77, r = 0.63, r = 0.56). The low RFOs genotypes identified in this study are particularly valuable in developing new black gram varieties for food and feed with improved digestibility and functionality.
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