High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds
2008
Todorović, Slađana | Giba, Zlatko | Bacic, Goran | Nikolić, Miroslav | Grubišić, Dragoljub
The effect of high Mn²⁺ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn²⁺ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000μM. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn²⁺ fraction decreased from 3% in the control (1μM Mn) to 0.35% and 0.15% in high Mn supply (1000μM and 10,000μM, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn²⁺ ions, which may generate ROS and affect seed viability, is alleviated.
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