Effects of combined expression of antifungal barley seed proteins in transgenic wheat on powdery mildew infection
2003
Bieri, S. | Potrykus, I. | Futterer, J.
Transgenic wheat plants (variety Frisal) constitutively expressing a number of potential antifungal proteins alone or in combinations were generated and tested for increased resistance to Blumeria graminis f.sp. tritici (powdery mildew) in a detached leaf infection assay. The most significative rate of protection was obtained with an apoplastic ribosome-inactivation protein from barley seed. Apoplastic Barnase was less efficient and individual plant lines harbouring a barley seed chitinase and beta-1,3-glucanase showed line specific phenotypes from increased resistance to increased susceptibility. Combination by crossing of three barley seed proteins did not lead to significant improvement of protection.
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