Differential extraction of radiocarbon associated with soil biomass and humus1
1999
Tsao, Chun-Wen | Bartha, Richard
To detect the humification of organic compounds in soil that bypasses biomass incorporation, selective extraction procedures for radiocarbon from soil biomass and humus were evaluated. Following the incubation of C-glucose and C-benzoate in soil, fumigation-0.5 M K2SO4 extraction and 0.15 M Na4P2O7 extraction selectively removed biomass-associated and humus-associated radiocarbon, respectively. Applying the recovery correction of 3.4 × to biomass and 3.5 × to humus, radiocarbon balances of 95 to 107% were obtained during a time window following the degradation of these substrates. Negligible overlap between the extractions renders the technique suitable for investigating the fate of organics that, through cometabolism, attain unusual radiocarbon distributions in soil.
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