Kinetic properties of (+)-catechin oxidation by a basic peroxidase isoenzyme from strawberries
1997
Lopez-Serrano, M. | Ros Barcelos, A.
The ability of a partially-heat stable strawberry (Fragaria X ananassa) peroxidase to oxidize (+)-catechin was studied and its oxidation followed the accepted model for peroxidase oxidations. Compound I (CoI) and compound II (CoII) were the main intermediates in the catalytic cycle. The reactivity of strawberry peroxidase with H2O2 [k1 = 2.63 micromolar-1 s-1] and with (+)-catechin [k3 = 0.57 micromolar-1 s-1] suggested that (+)-catechin is an excellent o-diphenol for CoII reduction. The strong cooperativity of this peroxidase isoenzyme indicates its great efficacy in oxidation of (+)-catechin at low H2O2 concentrations.
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