Investigation of apigenin-7-O-beta-glucoside hydrolysis by beta-glucosidase from almonds

Pekic, B.; Zekovic, Z.; Lepojevic, Z.

Investigation of apigenin-7-O-beta-glucoside hydrolysis by beta-glucosidase from almonds

1994

Pekic, B. | Zekovic, Z. | Lepojevic, Z.

The enzymatic transformation of apigenin-7-O-beta-glucoside into apigenin is effected using beta-glucosidase from almonds. Kinetic studies gave the following values Km = 7.53 X 10(-4)mol/dm3, vmax = 10.37 micromoles mg-1 min-1 and E1 = 65.21 kJ/mol when the substrate was pure apigenin-7-O-beta-glucoside, while in case of apigenin-7-O-beta-glucoside from dry extract of camomile ligulate flowers E2 = 85.57 kJ/mol.

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