Identification of a bisegmented double-stranded RNA virus (picobirnavirus) in calf faeces

Buzinaro, M.G.; Freitas, P.P.S.; Kisiellius, J.J.; Ueda, M.; Jerez, J.A.

Identification of a bisegmented double-stranded RNA virus (picobirnavirus) in calf faeces

2003

Buzinaro, M.G. | Freitas, P.P.S. | Kisiellius, J.J. | Ueda, M. | Jerez, J.A.

To determine the incidence of rotavirus infection among dairy herds in the State of São Paulo, Brazil, 576 faecal samples obtained from calves aged 1-45 days with and without diarrhoea, reared on 63 dairy cattle farms, were analyzed. Polyacrylamide gel electrophoresis (PAGE) identified 28 samples positive for group A rotavirus, while four samples, two diarrhoeic and two non-diarrhoeic, showed a bisegmented genome with a typical picobirnavirus pattern. Electron microscopy revealed spherical virus particles with a diameter of 37 nm and without a defined surface structure. The present study is the first report of a bisegmented virus identified in cattle in Brazil.

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