Reconstitution of allophycocyanin from Mastigocladus laminosus with isolated linker polypeptide
1993
Gottschalk, L. | Lottspeich, F. | Scheer, H.
The core linker polypeptide Lc(8.9) was isolated from Mastigocladus laminosus and purified on a preparative scale. A method for the reconstitution of allophycocyanin (AP)-linker complexes from isolated polypeptides was developed. The complex (alpha(AP)beta(AP))3 . Lc(8.9) was reconstituted and compared to (alpha(AP)beta(AP)) and (alpha(AP)beta(AP))3 by sucrose density gradient ultracentrifugation, absorption, fluorescence emission and circular dichroism spectroscopy. Differences in the spectra of reconstituted and of directly isolated AP complexes are discussed.
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