Improved photothermal therapy of brain cancer cells and photogeneration of reactive oxygen species by biotin conjugated gold photoactive nanoparticles
2021
He, Yuqing | Gao, Qiang | Lv, Chenglin | Liu, Lijun
Herein, we report on the design and development of functionalized acrylic polymeric nanoparticles with Spiropyrans (SPs) and imidazole moieties via superficial polymerizations. Then, Au³⁺ ions were immobilized and reduced on their surface to obtain photoresponsive gold-decorated polymer nanoparticles(Au-NPs). The synthesized Au-NPs were surface adapted with biotin as specific targeting tumor penetration cells and enhance the intercellular uptake through the endocytosis. FT-IR (Fourier-transform Infrared Spectroscopy), UV–Vis (Ultra Violet-Visible Spectrophotometer), EDS (Energy Dispersive X-Ray Spectroscopy), SEM (Scanning Electron Microscope) and HR-TEM (High-resolution transmission electron microscopy) descriptions were engaged to illustrate their spectral analysis and morphological examinations of Bt@Au-NPs. Fluorescence microscopy images of cellular uptake descriptions and ICP-MS (Inductively coupled plasma mass spectrometry) investigation established the cell lines labeling ability and enhanced targetting efficacy of biotin-conjugated Au-NPs (Bt@Au-NPs) toward C6 glioma cells (brain cancer cells) with 72.5% cellular uptake relative to 30.2% for non-conjugated lone. These were further established through intracellular ROS examinations and in vitro cytotoxicity investigation on the C6 glioma cell line. The solid surface plasmon absorptions of the Au-NPs and Bt@Au-NPs providing raised photothermal therapy under UV irradiation. The synthesized multifunctional Bt@Au-NPs with an inclusive combination of potential resources presented encouraging nanoprobe with targeting capability, improved photodynamic and photothermal cancer therapy.
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