Low melting point pyridinium ionic liquid pretreatment for enhancing enzymatic saccharification of cellulosic biomass
2013
Uju, | Nakamoto, Aya | Shoda, Yasuhiro | Goto, Masahiro | Tokuhara, Wataru | Noritake, Yoshiyuki | Katahira, Satoshi | Ishida, Nobuhiro | Ogino, Chiaki | Kamiya, Noriho
The potential of 1-hexylpyridinium chloride ([Hpy][Cl]), to pretreat cellulosic feedstocks was investigated using microcrystalline cellulose (Avicel) and Bagasse at 80°C or 100°C. Short [Hpy][Cl] pretreatments, <30min, at lower temperature accelerate subsequent enzymatic saccharification of Avicel. Over 95% conversion of pretreated Avicel to glucose was attained after 24h enzymatic saccharification under optimal conditions, whereas regenerated Bagasse showed 1–3-fold higher conversion than untreated biomass. FT-IR analysis of both Avicel and Bagasse samples pretreated with [Hpy][Cl] or 1-ethyl-3-methyimidazolium acetate ([Emim][OAc]) revealed that these ionic liquids behaved differently during pretreatment. [Hpy][Cl] pretreatment for an extended duration (180min) released mono- and disaccharides without using cellulase enzymes, suggesting [Hpy][Cl] has capability for direct saccharification of cellulosic feedstocks. On the basis of the results obtained, [Hpy][Cl] pretreatment enhanced initial reaction rates in enzymatic saccharification by either crystalline polymorphic alteration of cellulose or partial degradation of the crystalline cellulosic fraction in biomass.
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