The effect of light source and gelling agent on micropropagation of Rosa damascena Mill. and Rhynchostylis retusa (L.) Bl
2003
Anil Kumar, | Palni, L. M. S.
The effects of two gelling agents (agar or phytagel) and two light sources (cool fluorescent light or photosynthetically active radiation) on micropropagation of Rosa damascena Mill. and Rhynchostylis retusa (L.) Bl. were studied. Enhanced growth and better shoot proliferation was observed in cultures grown on medium gelled with phytagel (0.22%, w/v) and supplied with photosynthetically active radiation (PAR) as compared with cultures grown on medium gelled with agar (0.8%, w/v) and maintained under cool fluorescent lights (CFL). Direct protocorm-like bodies (PLBs) differentiated from the roots of regenerated plantlets of R. retusa on MS medium supplemented with 2.5 μM BA when the cultures were kept under PAR. While PLB differentiation was observed from the leaves of similar cultures incubated under CFL, better response was observed in cultures grown on medium containing phytagel. Differences were also recorded in respect of some physiological parameters, e.g. the relative water content, osmotic potential of the cell sap, chlorophyll content as well as dry weight of cultures grown under different conditions. Rooting was much more pronounced in microshoots of R. damascena when grown on medium gelled with agar and kept under PAR, and the number of roots per rooted shoot was also higher in agar gelled medium as compared to phytagel containing medium. In general, the plantlets raised under PAR showed higher survival (70% in agar and 80% in media gelled with phytagel) and better ex vitro growth than those cultured under CFL. It is suggested that a combination of PAR and phytagel is a useful option for micropropagation of these plants.
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