Water content, lipid deposition, and (+)-abscisic acid content in developing white spruce seeds
1999
Carrier, D.J. | Kendall, E.J. | Bock, C.A. | Cunningham, J.E. | Dunstan, D.I.
Whole seeds, and isolated megagametophytes, seed at coats and embryos from white spruce [Picea glauca (Moench) Voss] were used to monitor water distribution by magnetic resonance microimaging, lipid composition by gas chromatography, and (+)-abscisic acid content ((+)-ABA) by enzyme-linked immunosorbent assay, during seed development. The highest (+)-ABA content was detected in the female gametophytic tissue, this at 11 d after pollination (DAP). Reserve deposition was evident up to 59 DAP in the female gametophyte and in the developing embryo. The maturation drying period occurred between 59 DAP and 93 DAP. At 59 DAP, the seed contained 24% water (g(water)/g(wet weight)), and within a 34 d period, the water content declined to 6% water (g(water/g(wet weight)). (+)-ABA concentrations in developing seeds varied between 10 and 31 micromolar. The decrease in water content accounted for the higher (+)-ABA concentrations.
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