Trichoderma viride Tv-1511 Colonizes Arabidopsis Leaves and Promotes Arabidopsis Growth by Modulating the MAP Kinase 6-Mediated Activation of Plasma Membrane H+-ATPase
2020
Guo, Kai | Sui, Yonghui | Li, Zhe | Huang, Yanhua | Zhang, Hao
Plant growth is affected by a plethora of environmental factors including microorganisms. Trichoderma species are free-living fungi which are beneficial to plant by stimulating growth and disease resistance of plant, not only in the rhizosphere but also in the aerial parts. Here, we investigated the role of MAPK-mediated activation of plasma membrane (PM) H⁺-ATPase in regulating the leaf colonization of Trichoderma viride Tv-1511 and its growth-promoting effects in Arabidopsis. T. viride Tv-1511 colonized in the leaves of wild-type Arabidopsis leaves and induced the growth-enhanced phenotypes including increased biomass production, elevated nutrient uptake and stimulated leaf and seedling development. PM H⁺-ATPase plays an important role in the plant development and environmental responses. Results showed that PM H⁺-ATPase activity and expression of AHA1 gene was enhanced in T. viride Tv-1511-inoculated Arabidopsis. AHA1-deficiency inhibited the colonization of T. viride Tv-1511 in Arabidopsis leaves, and mutants in AHA1 gene were found to reduce the growth-promoting and developmental effects of T. viride Tv-1511 inoculation. Furthermore, MAP kinase 6 (MPK6) was determined to be activated by T. viride Tv-1511 inoculation, and involved in the leaf colonization and growth promotion induced by T. viride Tv-1511 by regulating the elevated expression and activity of PM H⁺-ATPase in Arabidopsis inoculated by T. viride Tv-1511. Taken all, our results highlighted the important role of MPK6-mediated increase of PM H⁺-ATPase expression and activity in the regulation of leaf colonization and plant growth promotion of T. viride Tv-1511.
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