Transposon tagging in rice
1997
Izawa, T. | Ohnishik, T. | Nakano, T. | Ishida, N. | Enoki, H. | Hashimoto, H. | Itoh, K. | Terada, R. | Wu, C. | Miyazaki, C.
To develop an efficient gene isolation method for rice we introduced the maize Ac/Ds system into rice. Extensive analysis of their behavior in rice for several generations indicated that Ac and Ds in the presence of Ac transposase gene actively transpose in rice. A wide spectrum of mutations affecting growth, morphogenesis, flowering time and disease resistance have been obtained in the population carrying Ac/Ds and some of them were genetically analyzed. Main efforts are currently being made to isolate genes responsible these mutations. In addition, a number of Ac/Ds were mapped on chromosomes and mapped elements will be used in the future for directed tagging of genes with known chromosomal positions.
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