Passive Ca²⁺ overload in H9c2 cardiac myoblasts: Assessment of cellular damage and cytosolic Ca²⁺ transients
2011
Soler, Fernando | Lax, Antonio | Carmen Asensio, M. | Pascual-Figal, Domingo | Fernández-Belda, Francisco
Increase of resting Ca²⁺ levels and amplitude of vasopressin-induced Ca²⁺ transients were observed when cells in serum-free medium were exposed to 5mM Ca²⁺ for 2h. Small effect on cell viability was also observed. A rapid cytotoxic effect was developed in the presence of 10mM Ca²⁺ and absence of serum. However, cells exposed to 10mM Ca²⁺ in the presence of serum were protected from damage for at least 2days. Resting Ca²⁺ levels and cytosolic Ca²⁺ transients in serum-containing medium with 10mM Ca²⁺ displayed lower increases and a tendency to recover control values. When serum was absent, cells preincubated with 10mM Ca²⁺ were more sensitive to thapsigargin-induced damage than cells preincubated with lower Ca²⁺. The sensitivity was similar when serum was present. Tolerance to high Ca²⁺ in the presence of serum was linked to potentiation of the mitochondrial Ca²⁺ entry to decrease the sarcoplasmic reticulum Ca²⁺ overload.
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