Micropropagation of Citrus halimii--an endangered species of South-east Asia
1997
Normah, M.N. | Hamidah, S. | Ghani, F.D.
A successful system of direct organogenesis is described for the wild citrus tree, Citrus halimii Stone which used in vitro seedling explants cultured on Murashige and Skoog medium supplemented with 0.4-11.1 micromolar 6-benzyladenine. Hypocotyl was the best explant for multiple shoots regeneration. Maximum number of shoots was obtained on medium with 2.2-11.1 micromolar 6-benzyladenine. Rooting of regenerated shoots was best on Murashige and Skoog medium supplemented with 2.7 micromolar alpha-naphthalenacetic acid. In vitro and ex vitro rooted plantlets survived (on average 83.3%) after being transferred to the soil mixture consisting of soil, sand and organic material (1:1:1) and kept in the glasshouse.
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