Isolation and identification of new compound, 2,7″-phloroglucinol-6,6′-bieckol from brown algae, Ecklonia cava and its antioxidant effect
2012
Kang, Sung-Myung | Heo, Soo-jin | Kim, Kil-Nam | Lee, Seung-Hong | Jeon, You-Jin
Brown algae, Ecklonia cava has been regarded as a useful material in the prevention or treatment of several human diseases in Jeju Island, Korea. In this study, we investigated its antioxidant activity, and isolated a new antioxidative compound, which was identified as 2,7″-phloroglucinol-6,6′-bieckol (PHB) on the basis of LC/MS, as well as ¹H and ¹³C NMR spectroscopy results. The antioxidant activity of PHB was evaluated via several in vitro assays, such as radical scavenging activities using electron spin resonance spectrometry, intracellular reactive oxygen species scavenging activity, and DNA damage assays. PHB evidenced profound scavenging activities on DPPH, alkyl, hydroxyl, and superoxide radicals (IC₅₀: 0.51, 2.07, 75.64 and 57.19μM for DPPH, alkyl, hydroxyl and superoxide radicals, respectively). The antioxidant activity of PHB was higher than that of ascorbic acid. Furthermore, PHB effectively inhibited H₂O₂-induced DNA damage. The results of this study indicate that PHB may prove useful as a novel natural marine antioxidant.
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