Reduction of aflatoxins by Rhizopus oryzae and Trichoderma reesei
2014
Hackbart, H. C. S. | Machado, A. R. | Christ-Ribeiro, A. | Prietto, L. | Badiale-Furlong, E.
This study evaluated the ability of the microorganisms Rhizopus oryzae (CCT7560) and Trichoderma reesei (QM9414), producers of generally recognized as safe (GRAS) enzymes, to reduce the level of aflatoxins B₁, B₂, G₁, G₂, and M₁. The variables considered to the screening were the initial number of spores in the inoculum and the culture time. The culture was conducted in contaminated 4 % potato dextrose agar (PDA) medium, and the residual mycotoxins were determined every 24 h by HPLC-FL. The fungus R. oryzae has reduced aflatoxins B₁, B₂, and G₁in the 96 h and aflatoxins M₁and G₂in the range of 120 h of culture by approximately 100 %. The fungus T. reesei has reduced aflatoxins B₁, B₂, and M₁in the 96 h and aflatoxin G₁in the range of 120 h of culture by approximately 100 %. The highest reduction occurred in the middle of R. oryzae culture.
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