An apple atp9 pseudogene is maintained at high copy number in ‘Golden Delicious’-type mitochondria but is present substoichiometrically in ‘Delicious’-type mitochondria
2012
Kato, Sumie | Kitazaki, Kazuyoshi | Wakatsuki, Akihito | Kishima, Yuji | Mikami, Tetsuo
In this study, the mitochondrial atp9 gene sequence of an apple cultivar ‘Golden Delicious’ was found to exist in one intact version and two truncated versions (termed φatp9-1 and φatp9-2). Interestingly, the φatp9-1 sequence is maintained at high copy number in the six ‘Golden Delicious’-cytotype cultivars examined but present substoichiometrically in eight ‘Delicious’-cytotype cultivars. Our data also suggest that φatp9-1 originated in a homologous recombination event mediated by the short repeat in a common ancestral mitochondrial genome of ‘Golden Delicious’ and ‘Delicious’, and was preferentially amplified in an evolutionary lineage that gave rise to the ‘Golden Delicious’-type genome. On the other hand, φatp9-2 was revealed to be present in high abundance in all 14 cultivars examined.
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