Mycobacterium paratuberculosis antigen D: characterization and evidence that it is a bacterioferritin
1991
Brooks, B.W. | Young, N.M. | Watson, D.C. | Robertson, R.H. | Sugden, E.A. | Nielsen, K.H. | Becker, S.A.W.E.
By using a combination of agarose and polyacrylamide gel electrophoresis, Mycobacterium paratuberculosis antigen D was resolved from a crude sonicated preparation of the organism and characterized as a component with a molecular mass of approximately 400,000 Da. While this component was composed mainly of protein, with unusually high proportions of glutamic acid and leucine, it was resistant to digestion with a number of proteolytic enzymes. Structural detail revealed by electron microscopy, amino acid sequence data, and the demonstration of a Soret band in its absorption spectrum indicated that antigen D was similar to an Escherichia coli bacterioferritin.
显示更多 [+] 显示较少 [-]