Production of L-lactic acid by Rhizopus oryzae under oxygen limiting conditions

Skory, C.D.; Freer, S.N.; Bothast, R.J.

Production of L-lactic acid by Rhizopus oryzae under oxygen limiting conditions

1998

Skory, C.D. | Freer, S.N. | Bothast, R.J.

During aerobic growth, Rhizopus oryzae produces L-lactic acid from lactate dehydrogenase mediated reduction of pyruvate, while O2 limiting conditions yield primarily ethanol. A mutant was isolated that expressed only 5% of the wild type alcohol dehydrogenase activity under O2 limiting conditions and produced nearly 40 g lactic acid/l in 70 h. This is almost a ten-fold increase in lactic acid production when compared to the parent strain.

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