Gene II product of an aphid-nontransmissible isolate of cauliflower mosaic virus expressed in a baculovirus system possesses aphid transmission factor activity
1993
Blanc, S. | Cerutti, M. | Chaabihi, H. | Louis, C. | Devauchelle, G. | Hull, R.
A new baculovirus expression system was used to express the gene II which encodes the 18-kDa aphid transmission factor (ATF) protein of two isolates of cauliflower mosaic virus (CaMV), the aphid-transmissible (AT+) Cabb B-Jl and the aphid-nontransmissible (AT-) CM1841 isolates. ATF activity was shown to be associated with the baculovirus expression product of CaMV gene II from both the AT+ and the AT- isolates. No differences could be detected in the stability of the P18s from AT+ and AT- isolates expressed in the baculovirus system. These results indicate that the AT-phenotype of CM1841 isolate might not be due to a defective P18 but to an insufficient concentration of it in infected plant tissue.
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