A mutant of Arabidopsis thaliana lacking the ability to transport glucose across the chloroplast envelope
1994
Trethewey, R.N. | Ap Rees, T.
At the end of a 12-h day leaves of the mutant of Arabidopsis thaliana L., TC265, contained 4-5 times more starch than those of the wild type. During a subsequent 12-h night the decline in the starch content of the leaves of the mutant was at least 50% of that of the wild-type leaves. Starch labelled in the light in a 30-min pulse in (14)CO2 was rapidly broken down in a subsequent 12-h chase in the dark in air in the leaves of both mutant and wild type. Chloroplasts from leaves of the wild type took up [(32)P]Pi and [U-(14)C]glucose at 12 and 1.6 micromole/h per mg of chlorophyll respectively; chloroplasts from the mutant showed a similar rate for [(32)P]Pi but no uptake of [U-(14)C]glucose. The glucose content of freshly isolated chloroplasts from the mutant was twice that of chloroplasts from the wild type; this difference was accentuated when the isolated chloroplasts were incubated in the dark. SDS/PAGE of preparations of chloroplast envelopes showed that those from the mutant were deficient in a protein band of approximate molecular mass 40 kDa. It is suggested that in mutant TC265 the primary lesion is in a hexose transporter in the chloroplast envelope, and that this transporter moves the products of starch breakdown that are destined for sucrose synthesis from the chloroplast to the cytosol.
显示更多 [+] 显示较少 [-]