Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts [Erratum: July 2022, Vol.227(6), p.2229]
2021
Štefančík, Michal | Válková, Lucie | Veverková, Jana | Balvan, Jan | Vičar, Tomáš | Babula, Petr | Mašek, Josef | Kulich, Pavel | Pávková Goldbergová, Monika
Titanium-based alloys have established a crucial role in implantology. As material deteriorates overtime, nanoparticles of TiO₂ and Ni are released. This study is focused on the impact of TiO₂ and Ni nanoparticles with size of 100 nm on cytoskeletal and adhesive changes in human physiological and osteoarthritic osteoblasts. The impact of nanoparticles with concentration of 1.5 ng/mL on actin and tubulin expression and gene expression of FAK and ICAM-1 was studied. The cell size and actin expression of physiological osteoblasts decreased in presence of Ni nanoparticles, while TiO₂ nanoparticles caused increase in cell size and actin expression. Both cell lines expressed more FAK as a response to TiO₂ nanoparticles. ICAM-1 gene was overexpressed in both cell lines as a reaction to both types of nanoparticles. The presented study shows a crucial role of Ni and TiO₂ nanoparticles in human osteoblast cytoskeletal and adhesive changes, especially connected with the osteoarthritic cells. Graphical abstract
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