The presence of concanavalin A and canatoxin in Canavalia ensiformis DC tissue culture
1993
Sato, A. | Barcellos, G.B.S. | Riedel, E.C. | Carneiro, J.A. | Carlini, C.R. | Esquibel, M.A.
Isolated embryos, cotyledons and embryos plus a fragment of cotyledon from seeds of Canavalia ensiformis (jack bean) were cultured in vitro. Concanavalin A and canatoxin cross-reactive material were detected by double immunodiffusion tests. Canatoxin was detectable until 30 days in cultures of embryos, embryos plus cotyledons and hypocotyls. Concanavalin A was also present in all cultures being detected until 90 days in cultures treated with 6-benzylaminopurine. No concanavalin A was detected in root cultures. Concanavalin A was present in cell suspensions until 45 days of culture; the culture medium contained neither concanavalin A nor canatoxin. Tissue cultures thus can produce Con A and CNTX and will be an important research tool for studying the biosynthesis of such substances.
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