Epidermal and guard cell interactions on stomatal aperture in epidermal strips and intact leaves | Epidermal and guard cell interactions on stomatal aperture in epidermal strips and intact leaves [Vicia faba, broadbeans]
1983
Spence, R.D. | Sharpe, P.J.H. | Powell, R.D. | Rogers, C.A.
Despite the observation first made by von Mohl in 1856, that epidermal cells greatly influence stomatal aperture, subsequent studies have failed to pay adequate attention to epidermal cell viability or to quantify the degree of its influence on aperture in epidermal strips and leaf sections. Using Vicia faba strips and leaf sections we found the following: (i) a non-linear relationship between aperture and guard cell contact with live epidermal cells; (ii) epidermal cell viability on isolated strips had a threshold at about 25 °C; (iii) epidermal strips with dead epidermal cells had wider apertures and lower variability than strips with live cells or intact leaf sections; (iv) after epidermal cell viability was accounted for, stomatal apertures showed no significant differences between isolated strips or strips removed from leaf sections treated in the same manner; (v) highly variable apertures appeared to be the normal condition of the intact leaf. Caution should therefore be used in interpreting stomatal behaviour from epidermal strips without first taking into account mechanical interactions between the guard and surrounding epidermal cells.
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