The Isoflavonoid Daidzein Attenuates the Oxidative Damage Induced by Polychlorinated Biphenyls on Cultured Chicken Testicular Cells
2007
Mi, Y.L. | Zhang, C.Q. | Zeng, W.D. | Liu, J.X. | Liu, H.Y.
The soy isoflavonoid daidzein (DAI) is one of the most abundant phenolic compounds in the human diet and in animal feedstuffs. Daidzein possesses a wide spectrum of physiological and pharmacological functions related to human health. The aim of this study was to evaluate the effect of DAI on oxidative damage induced by a potential carcinogen, polychlorinated biphenyl. Testicular cells were dispersed from 18-d-old chicken embryos and exposed to DAI alone or in combination with a mixture of polychlorinated biphenyls, Aroclor 1254 (A1254), in culture. Oxidative damage was estimated by measuring the lipid peroxidation and superoxide dismutase activities and glutathione content. Results showed that DAI (10 μg/mL) increased germ cell numbers, which were inhibited by cotreatment with the estrogen receptor antagonist tamoxifen at 0.1 μg/mL. Exposure to A1254 (10 μg/mL) reduced superoxide dismutase activity and glutathione content but increased lipid peroxidation significantly. However, simultaneous supplementation with 10 μg/mL of DAI restored these parameters. The above results indicated that DAI may exert weak estrogenic activity, and more important, that DAI may display an antioxidant effect to prevent oxidative damage induced by the oxidant A1254.
显示更多 [+] 显示较少 [-]