Use of denatured radioalbumin for determination of trypsin and chymotrypsin inhibitors in different plant seeds

Blahovec, J.

Use of denatured radioalbumin for determination of trypsin and chymotrypsin inhibitors in different plant seeds

1991

Blahovec, J.

The procedure for determination of trypsin and chymotrypsin inhibitors with urea-denatured albumin labeled by 125I is described. The content of both types of inhibitory activity has been determined in crude extracts of soybean, bean, lentil, pea, horse bean, maize, and 20 pea cultivars. The method is sufficiently sensitive, reliable, and particularly suitable when estimations must be done in crude plant extract with low inhibitory activity.

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