Dynamic monitoring and quantitative characterization of intracellular H2O2 content by using SERS based boric acid nanoprobe
2020
Cheng, Wendai | Tang, Ping | He, Xuanmeng | Xing, Xinyue | Xing, Meishuang | Lu, Liupeng | Ju, Peng | Wang, Yi | Liu, Shengde | Lu, Xiaoxu | Zhong, Liyun
Quantitative characterization of intracellular H₂O₂ content, which is still difficult by the conventional biochemical methods due to the lack of real-time and non-invasive technique of single cell measurement, is a useful solution for cell state assessment. Based on the surface enhanced Raman scattering (SERS), we construct a novel boric acid (BA) nanoprobe to perform quantitative characterization of H₂O₂ content, in which the p-thiol benzene boric acid (4-MPBA) reporter molecule modified with gold nanorods (AuNRs) is employed for Raman signal enhancement. The achieved result demonstrates obvious advantages of the synthesized AuNRs/4-MPBA/BA nanoprobe in measurement sensitivity of H₂O₂ content. Importantly, this AuNRs/4-MPBA/BA nanoprobe will provide a powerful tool for dynamic monitoring and quantitative characterization of intracellular H₂O₂ content during cell apoptosis or other cell growth processes, and then achieve important reference data for studying the corresponding molecular mechanism.
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