Secretion of N-terminal domain of α-dystroglycan in cerebrospinal fluid
2011
Saito, Fumiaki | Saito-Arai, Yuko | Nakamura-Okuma, Ayami | Ikeda, Miki | Hagiwara, Hiroki | Masaki, Toshihiro | Shimizu, Teruo | Matsumura, Kiichiro
α-Dystroglycan (α-DG) plays crucial roles in maintaining the stability of cells. We demonstrated previously that the N-terminal domain of α-DG (α-DG-N) is secreted by cultured cells into the culture medium. In the present study, to clarify its function in vivo, we generated a monoclonal antibody against α-DG-N and investigated the secretion of α-DG-N in human cerebrospinal fluid (CSF). Interestingly, we found that a considerable amount of α-DG-N was present in CSF. α-DG-N in CSF was a sialylated glycoprotein with both N- and O-linked glycan. These observations suggest that secreted α-DG-N may be transported via CSF and have yet unidentified effects on the nervous system.
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