Purification of a lipase from Acinetobacter calcoaceticus AAC323-1 by hydrophobic-interaction methods
1996
Bompensieri, S. | Gonzalez, R. | Kok, R. | Miranda, M.V. | Nutgeren-Roodzant, I. | Hellingwerf, K.J. | Cascone, O. | Nudel, B.C.
The performance of hydrophobic-interaction chromatography (HIC) for the purification of Acinetobacter calcoaceticus AAC323- 1 lipase was compared with that of various aqueous two-phase systems. While a 42% lipase yield with a purification factor of 140 could be recovered by HIC, higher yields were achieved by using aqueous two-phase systems, either those formed by poly(ethylene glycol) and dextran or those based upon the use of a detergent. Triton X-114-based aqueous two-phase partition showed the best performance, with a yield of 81% and a purification factor of 68. Further detergent removal was easily achieved with an adsorbent, with no significant decrease in yields. Owing to its simplicity, the method should be easy to scale-up.
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