Development of self-incompatible Brassica napus. I. Introgression of S-alleles from Brassica oleracea through interspecific hybridization
2003
Ripley, V.L. | Beversdorf, W.D.
Cultivars in Brassica napus var. oleifera, a self-pollinating, self-compatible species, have traditionally been developed as open-pollinated lines or populations. Significant yield gains in this species have been realized through the exploitation of heterosis. Commercial hybrid production has been possible as a result of the development of a number of pollination control systems. Self-incompatibility was transferred from B. oleracea var. italica to B. napus var. oleifera through interspecific hybridization. The response to interspecific pollination, as measured by pod elongation and initial stages of ovule development, was genotype dependent, and two highly responsive B. napus genotypes were identified. Embryo rescue was used to produce the interspecific hybrids. Isoelectric focusing of stigma proteins was used to identify S-alleles in the interspecific hybrids to facilitate backcrossing. Segregation of the S-locus through a series of back-crosses to B. napus was complicated by aneuploidy; however, the S-locus was found to segregate as a single gene. Usefulness of B. oleracea as a source of S-alleles for pollination control in B. napus is discussed.
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