Label‐free proteome of water buffalo (Bubalus bubalis) seminal plasma
2018
Brito, Mayara F. | Auler, Patrícia A. | Tavares, Guilherme C. | Rezende, Cristiana P. | Almeida, Gabriel M. F. | Pereira, Felipe L. | Leal, Carlos A. G. | Moura, Arlindo de Alencar | Figueiredo, Henrique C. P. | Henry, Marc
The study aimed to describe the Bubalus bubalis seminal plasma proteome using a label‐free shotgun UDMSᴱ approach. A total of 859 nonredundant proteins were identified across five biological replicates with stringent identification. Proteins specifically related to sperm maturation and protection, capacitation, fertilization and metabolic activity were detected in the buffalo seminal fluid. In conclusion, we provide a comprehensive proteomic profile of buffalo seminal plasma, which establishes a foundation for further studies designed to understand regulation of sperm function and discovery of novel biomarkers for fertility. MS data are available in the ProteomeXchange with identifier PXD003728.
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