Photoconjugation of an Fc-Specific Peptide Enables Efficient DAR 2 Antibody–Drug Conjugate Formation

Lee, TaeJin; Kim, Ju Hwan; Kwon, Se Jeong; Park, Sun Hee; Kim, Jinyoung; Kang, Hyo Jin; Chung, Sang J.

Photoconjugation of an Fc-Specific Peptide Enables Efficient DAR 2 Antibody–Drug Conjugate Formation

2020

Antibody–drug conjugates (ADCs) in current clinical usage have been constructed via the nonspecific conjugation of drugs to antibodies, rendering the manufacturing processes difficult. In this study, a high-affinity IgG Fc-binding peptide equipped with a photoreactive amino acid was developed and successfully conjugated to Glu-382 of trastuzumab in a site-specific manner. The resulting conjugate was employed to generate a DAR 2 ADC product using click chemistry.

显示更多 [+]