Rapid detection of a small number of viable cells by the fluorescent glucose method
2002
Matsuoka, H. | Kurokawa, T. | Oishi, K. | Saito, M.
A fluorescent glucose, 2NBDG, was rapidly consumed by 4 different species of microorganisms: Escherichia coli, Lactobacillus acidophilus, Saccharomyces cerevisiae, and Candida tropicalis. E. coli and L. acidophilus became fluorescent enough for microscopic observation within 1 min, while S. cerevisiae and C. tropicalis became fluorescent in a longer time span. All of the 14 coliforms that were isolated from various foods consumed 2NBDG and became fluorescent within 1 min. Therefore, 2NBDG is expected to be a useful indicator of viable cells irrespective of species as long as they could assimilate glucose. This 2NBDG method is valid in counting small amount of cells, such as 10-100 cells, accurately and carefully. Also, the 2NBDG method was successfully applied to the rapid detection of a small number of E. coli in milk. The feasibility of the 2NBDG method is discussed from the viewpoint of food safety control.
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