Shorter abstinence decreases sperm deoxyribonucleic acid fragmentation in ejaculate
2011
Gosálvez, Jaime | González-Martínez, Mercedes | López-Fernández, Carmen | Fernández, José L. | Sánchez-Martín, Pascual
OBJECTIVE: To evaluate the relationship between duration of sexual abstinence and sperm selection on sperm DNA fragmentation (SDF). DESIGN: Prospective study based on normozoospermic individuals. SETTING: Fertility and IVF unit and university unit for research. PATIENT(S): Two cohorts of normozoospermic individuals: 21 men (aged 25–35 years) attending a clinic and with clearly adverse female factors; and a group of 12 selected donors (aged 20–25 years). INTERVENTION(S): SDF assessment using the sperm chromatin the dispersión test (Halosperm) in two cohorts of normozoospermic men. MAIN OUTCOME MEASURE(S): SDF assessment after 24 hours of abstinence with recurrent ejaculation (one every 24 hours) using neat sperm samples; and SDF assessment before and after sperm selection with abstinence of 3 hours. RESULT(S): Lower baseline levels of SDF were observed after shorter periods of abstinence between ejaculations (24 hours and 3 hours) than those recommended. This effect is much more marked after quick repetitive ejaculation (3 hours of abstinence) and sperm selection. CONCLUSION(S): The present results challenge the role of abstinence in current male infertility treatments and suggest that SDF can be efficiently reduced by a biological practice consisting of short-term recurrent ejaculation coupled with effective sperm selection.
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