Selection of a Field Population of Diamondback Moth (Lepidoptera: Plutellidae) with Acetamiprid Maintains, but Does Not Increase, Cross-Resistance to Pyrethroids
2007
Sayyed, A.H. | Crickmore, N.
Bioassays (at generation G 2) with a newly collected field population (designated CH4) of Plutella xylostella (L.) (Lepidoptera: Plutellidae) from farms in the Cameron Highlands, Malaysia, indicated resistance to acetamiprid, deltamethrin, lambda-cyhalothrin, and esfenvalerate. At G 3, the field-derived population was divided into two subpopulations, one subpopuplation was selected (G 3 to G 10) with acetamiprid (aceta-SEL), whereas the second subpopulation was left unselected (UNSEL). A significant reduction in the resistance ratio for each compound was observed in UNSEL at G 11, indicating that the observed resistance to each insecticide was unstable. For aceta-SEL, bioassays at G 11 found that selection with acetamiprid gave a resistance ratio of 409 compared with UNSEL. The LC 50 values for deltamethrin, lambda-cyhalothrin, and esfenvalerate to aceta-SEL were similar at both G 11 and G 2. This suggests that acetamiprid selection maintained the otherwise unstable resistance to these compounds in the aceta-SEL population. Logit regression analysis of F 1 reciprocal crosses between aceta-SEL and the susceptible Lab-UK indicated that resistance to acetamiprid was inherited as an autosomal, incompletely recessive ( D LC = 0.26) trait. Tests of monogenic inheritance and weight distribution suggested that resistance to acetamiprid was controlled by a single locus.
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