Screening of Anti-nonstructural Protein Antibodies against FMD Virus using 3AB3 Recombinant Protein-Based DIVA ELISA in Uttar Pradesh
2015
Singh, Rashmi | Singh, Ajay Pratap | Singh, Tripti | Kumar, Yogesh | Yadav, Sharad Kumar
Detection of antibodies against non-structural proteins (NSPs) of foot-and-mouth disease (FMD) virus helps in differentiation of FMD infected from vaccinated animals (DIVA). Among all the NSPs, 3AB3 NSP can be used to assess FMD sero-surveillance in bovine population. In the present study, a total of 10, 422 bovine serum samples from buffalo (n = 5, 038) and cattle (n = 5, 384) collected on the day of vaccination during XVI phase of vaccination in the year 2014 under FMD control programme (FMD-CP) from 65 districts of the state of Uttar Pradesh were tested by 3AB3-based NSP-Enzyme-Linked Immunosorbent Assay (ELISA). Overall, 18.62% (1, 941/10, 422) serum samples were found reactive to anti-3AB3 NSP antibodies with 19.71% (1, 061/5, 384) cattle and 17.47% (880/5, 038) buffalo serum samples positive. A region-wise analysis showed significantly high level of anti NSP-3AB3 antibodies in Bundelkhand region followed by Central and Western Uttar Pradesh, whereas Eastern Uttar Pradesh recorded lowest anti NSP-3AB3 reactivity.
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