4-Nonyphenol induced apoptosis and DNA damage in the African catfish Clarias gariepinus, the antioxidant role of Cydonia oblonga
2017
Sayed, Alaa El-Din H. | Hamed, Heba S.
The present study was planned to assess the toxic effects of sublethal concentration (0.1 mgl−1) 4-nonylphenol (4-NP) on serum biochemical parameters, liver lipid peroxidation (LPO) and the antioxidant enzymes of the African catfish Clarias gariepinus for 14 days and the ability of the quince leaf extract to alleviate the effects of (4-NP) on C. gariepinus. Fish were categorized into four groups: control, exposed to (0.1 mgl−1) (4-NP), exposed to (0.1 mgl−1) (4-NP) with adding quince leaf extract (10ml /30L water), and exposed to (0.1 mgl−1) (4-NP) with adding quince leaf extract (20ml /30L water). 4-NP exposure induced a significant (p<0.05) increase in the level of glucose, AST, ALT, creatinine, urea, uric acid, cholesterol, and G6PDH. As well as, the percentages of hepatic LPO level, DNA fragmentation, and apoptotic erythrocytes were significantly (p<0.05) elevated. A significant (p<0.05) decrease in alkaline phosphatase (ALP), total protein, albumin, globulin, total lipids, and LDH were also recorded. Liver enzyme activities (SOD, CAT, and TAC) were decreased. Adding the quince leaf extract into the water was able to reinstate the alterations in biochemical parameters, antioxidant biomarkers, apoptotic level and hepatic DNA damage induced by (4-NP) intoxication.
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