Fine mapping of a major QTL controlling panicle number in rice
2011
Zhu, Jinyan | Zhou, Yong | Liu, Yanhua | Wang, Zhongde | Tang, Zaixiang | Yi, Chuandeng | Tang, Shuzhu | Gu, Minghong | Liang, Guohua
Rice panicle number per plant is a grain yield component that directly influences rice yield. The identification of the genes controlling panicle number will play a vital role in high-yield rice breeding. C3074, a chromosome segment substitution line containing an introgression segment of Nipponbare in the genetic background of Guangluai 4, had significantly fewer panicles. Quantitative trait locus (QTL) analysis in F2 and F3 generations derived from a cross between C3074 and Guangluai 4 revealed that qPN1, a major QTL locating on the long arm of chromosome 1, was responsible for this phenotypic variation. qPN1 also affected plant height, panicle length, grain number per panicle and grain yield per plant. Genetic analysis indicated that qPN1 acted as a single Mendelian factor, and that the allele from Nipponbare decreased the panicle number per plant. Fine mapping was carried out with a total of 1,429 recessive individuals from F2 and F3 generations, localizing the gene to a 34.4-kb region containing six annotated genes according to available sequence annotation databases. This information will be helpful for future identification and isolation of the candidate gene. The tightly linked markers that we have developed for qPN1 will be a useful tool in the marker-assisted selection of this gene in rice improvement programs.
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