RAPD and SCAR markers for powdery mildew resistance gene er in pea
2004
Janila, P. | Sharma, Binaya
In pea, a single recessive gene (er) on linkage group 6 confers resistance to powdery mildew caused by Erysiphe pisi. The present study aims to identify molecular markers linked to the er gene. Screening of the powdery mildew-resistant cultivar 'DMR11' and its susceptible near-isogenic line for polymorphism revealed linkage of two RAPD primers (OPO-02 and OPU-17) to the er gene and a sequence characterized polymorphic region (SCAR) primer, ScOPD-10(650) with er in a population of 83 F2 plants in the order: OPU-17 - er- ScOPD-10(650) - OPO-02. The markers ScOPD-10(650) and OPU-17 being coupled with the allele causing resistance would substantially increase the efficiency of marker-assisted selection in peabreeding for powdery mildew.
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