Venom of an aggressive African Theraphosidae (Scodra griseipes): milking the venom, a study of its toxicity and its characterization
1993
Celerier, M.L. | Paris, C. | Lange, C.
Two milking procedures, electric stimulation and lure-biting, are described and compared. The amount of crude Scodra griseipes venom collected in 3 years by lure-biting was 1670 mg and 1380 mg, respectively, for young females and adult males. The crude venom appears pure on the basis of UV-Vis spectra, which show no hemocyanin absorption bands. The toxicity and induced symptoms of these venoms in mice were evaluated by direct bites and also by LD50 [LD50 (young female venom, i.v.) = 8.1 mg/kg, LD50 (adult male venom, i.v.) > 9.5 mg/kg]. The venom from both sexes was compared by protein content, UV-Vis and 1H-NMR spectroscopies, polyacrylamide gel electrophoresis [SDS-PAGE] and size exclusion high performance liquid chromatography [SE-HPLC]. Differences were observed.
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